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The green fluorescent protein ( GFP ) is a protein composed of 238 amino acid residues that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is can be excited by the 488 nm laser line and is optimally detected at 510 nm.
The biggest advantage of GFP is that it can be heritable, depending on how it was introduced, allowing for continued study of cells and tissues it is expressed in. Visualizing GFP is noninvasive, requiring only illumination with blue light.
To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.

 GFP Biomedical Filter

Filter Type Fluoresence Filter Set
Coating Type Hard Coated
Part Number GFP-BP01A
Individual Filter  Excitation Filter  Emission Filter  Dichroic Mirror
BP-EX03A BP-EM03A BP-DM03A
CWL/FWHM 472/30nm 520/35nm 495nm
Angle of Incidence 0±5°  0±5°  45°
Transmission >80%  >80%  >90%
Size(mounted) Ø25mm x 5mm Ø25mm x 3.5mm Ø25.2mm x 35.6mm x 1.05mm
Wavelength Tolerance ±3nm
Blocking OD>5
Scratch-Dig 60-40
Substrate Fused Silica, B270
Compatible Microscopes Most common Nikon, Olympus, and Zeiss Microscopes
Compatible Fluorophore 3,3'-diheptyloxacarbocyanine iodide (DIOC7(3)),CopGFP,DIOC7(3) (3,3'-diheptyloxacarbocyanine iodide),EmGFP (Emerald),GFP (S65A),GFP (S65C),GFP (S65L).
Fluorescence Image Gallery

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