30025 Single band filter set FITC

Part Number:30025 FITC          

Fluorophore Displayed:Alexa Fluor® 488,Fluo-4,FITC (Fluorescein),SpectrumGreen,SYBR Green,GFP (EGFP),rsGFP, BODIPY, 5-5-FAM and Alexa Fluor 490

Individual filters and filter sets (no cube)These filters sets contain individual filters. 

Type Size Part Number AOI Price
Single Band Exciter φ25 x 5.0 mm 30025-BP475 $-
Single Band Emitter φ25 x 3.5 mm 30025-BP530 $-
Single Dichroic Mirror 36 x 25.7 x 1.05mm 30025-DM500 45° $-
Total:

High Contrast FITC set in life science field which contains exciter, emitter and dichroic mirror designed to fit in standard filter cubes from the leading microscope brand, suitable for LED light sources. It is optimized for fluorescein isothiocyanate (FITC) and other fluorophores such as rsGFP, BODIPY, 5-5-FAM, Fluo-4, and Alexa Fluor 490.

Optical Specifications

Filter Type30025 Single band filter set FITC
Coating TypeHard Coating
Angle of Incidence0°±5°
Exciter475nm
Transmission>85%
Central BlockingOD>5
Cutoff BlockingOD>3
Blocking Band300~1100nm
Angle of Incidence0°±5°
Emitter530nm
Transmission>85%
Central BlockingOD>5
Cutoff BlockingOD>3
Blocking Band300~1100nm
Angle of Incidence45°±1°
Edge Wavelength500nm
ReflectionR>98%
TransmissionT>85%

General Filter Specifications

Diameter Dimensions25 mm
Diameter Tolerance (mounted)± 0.1 mm
EX Filter Thickness (mounted) 5.0 mm
EM Filter Thickness (mounted) 3.5 mm
Filter Thickness Tolerance (mounted)± 0.1 mm
Clear Aperture≥ 21 mm
DM Square Dimensions 36 x 25.7 x 1.05mm
DM Thickness(unmounted)1.05 mm
Thickness Tolerance (unmounted)± 0.1 mm
Flatnessλ/4
Parallelism30″
Scratch-Dig60-40
SubstratesFused Silica, B270
Compatible MicroscopesMost common Nikon, Olympus, Zeiss and Leica microscopes